Reinvestigation of DNA ligase I in axolotl andPleurodelesdevelopment
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چکیده
منابع مشابه
Reinvestigation of DNA ligase I in axolotl and Pleurodeles development.
We have recently shown that the exclusion process causing the replacement of DNA ligases II by DNA ligase I in amphibian eggs after fertilization does not occur in the case of Xenopus laevis [Hardy, S., Aoufouchi, S., Thiebaud, P., and Prigent, C., (1991) Nucleic Acids Res. 19, 701-705]. Since this result is in contradiction with the situation reported in axolotl and Pleurodeles we decided to r...
متن کاملControl of DNA ligase molecular forms in nucleocytoplasmic combinations of axolotl and Pleurodeles.
A light form of DNA ligase (EC 6.5.1.2), the only form present in oocytes of the axolotl (Ambystoma mexicanum), has been shown to be replaced by a heavy form of the enzyme when the egg enters cleavage. This early biochemical event has been assumed to rely on direct nuclear input. Sucrose gradient analysis permits discrimination between enzymes from axolotl and the sharp-ribbed salamander (Pleur...
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DNA-ligase activity in eukaryotic cells is carried out by two different molecular forms of the enzyme. This molecular duality, first described in mammals (Soderhall & Lindhal, 1973), has been reported in chicken (David, 1977), amphibians (Carre, Signoret, Lefresne & David, 1981), fish, sea urchin and crab (Signoret & David, 1986). Depending on the developmental stage and the tissue considered, ...
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PURPOSE Human DNA ligase I plays an essential role in DNA replication, recombination, and repair by catalyzing the formation of phosphodiester bonds between adjacent 5'-phosphoryl and 3'-hydroxyl termini at single breaks in duplex DNA molecules. DNA ligase I is responsible for the majority of DNA ligase activity present in proliferating cells among four different forms of DNA ligases, designate...
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Cell lines derived from seven patients with Bloom syndrome all contain a DNA ligase I with unusual properties. Six lines were shown to have a reduced level of this enzyme activity and the residual enzyme was anomalously heat-labile. The seventh line contained a dimeric rather than monomeric form of ligase I. Several cell lines representative of other inherited human syndromes have apparently no...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.16.4395